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Chromatographic performance and selectivity in HPCIC primarily depends upon the nature of the immobilised chelating reagent, or in certain cases on the relative combination of multiple functional groups attached to the surface of high-performance grade substrates. However, other properties of chelating stationary phases also influence chromatographic performance, including functional group charge effects, functional group distribution and surface density, substrate matrix effects such as porous structure, and the type of the material used, e.g. polymer or silica based. This Chapter describes the wide range of stationary phases applied within HPCIC and details their chromatographic performance and selectivity in relation to the above physical and chemical characteristics.

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