Depending on the delays between RF pulses and the relative phases and flip angles of these pulses, new spectral information can be obtained with a multiple-pulse sequence that would not be available from a spectrum obtained with a single RF pulse. This is illustrated here with a discussion of different one-dimensional pulse sequences. The first are two sequences designed respectively to measure T₁ and T₂ relaxation times. As discussed in this and other chapters, knowledge of T₁ values is important for the choice of acquisition parameters. The ‘spin-echo’ sequence for measurement of T₂ is important, mainly because spin-echo segments are important components of many other pulse sequences. The second types of pulse sequences that are discussed in this chapter are those for ¹³C spectral editing (i.e., sequences that can distinguish between different peaks in a ¹³C spectrum based on their number of attached protons). The relative merits of the different sequences are compared and we recommend what we regard as the best choice. The third types of sequences discussed here are those for solvent suppression, i.e., sequences designed to remove solvent peaks or other strong impurities peaks while having a minimal effect on solute peaks. Finally, sequences, called pure shift sequences, that give broadband ¹H decoupling in ¹H spectra and in some ¹H-detected 2D sequences are discussed.