In Situ Analysis of Cellular Functional Molecules
Chapter 2: In Situ Detection of Cell-surface Glycans
Published:14 Jan 2020
Special Collection: 2020 ebook collectionSeries: Detection Science
2020. "In Situ Detection of Cell-surface Glycans", In Situ Analysis of Cellular Functional Molecules, Huangxian Ju, Bo Tang, Lin Ding
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Glycosylation is an important type of chemical modification of proteins and lipids and plays essential roles in regulating their structures, properties and functions. Cell-surface glycans are closely related to the intercellular and cell-matrix interactions and mediate cell differentiation, proliferation and migration. The progression of a variety of diseases exhibits well-established relationships with changes in the structures and expression levels of cell-surface glycans. In this context, the in-depth study of cell-surface glycans has obvious implications for understanding the intricate biological roles of cell-surface glycans in pathological processes, developing new methods for clinical diagnosis and drug screening and further revealing the essence of life. Owing to the non-template synthesis processes, complex structures and sophisticated modifications, the in situ analysis of cell-surface glycans is challenging compared with protein and nucleic acid biosensing. This chapter outlines representative analytical techniques and research advances in cell-surface glycan analysis, including cell-surface glycan recognition and labelling techniques, global analysis, competition detection format and protein-specific imaging. Through dynamic tracking of cell-surface glycans, exploration of the correlation between glycan patterns and glycan-related biological processes can be achieved.