In Situ Analysis of Cellular Functional Molecules
Chapter 9: Imaging of the Tumour Microenvironment
Published:14 Jan 2020
Special Collection: 2020 ebook collectionSeries: Detection Science
2020. "Imaging of the Tumour Microenvironment", In Situ Analysis of Cellular Functional Molecules, Huangxian Ju, Bo Tang, Lin Ding
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Acidity and hypoxia are both pivotal features in diseases such as cardiovascular disease, inflammation, ischaemia, stroke and chronic lung disease. The intracellular microenvironment shows different pH values such as pH 7.2 in cytosol and pH 4.0–6.0 in lysosomes and endosomes; however, variations of pH exceeding 0.1–0.2 units will lead to various diseases and may even be life threatening. Hypoxia, particularly in tumours, triggers series of cases and therapy blockade. Therefore, monitoring of pH fluctuations and hypoxia will afford important information about disease analysis and diagnosis, which are of great significance in acquiring a deep understanding of various disordered pathophysiological processes and therapy results. Molecular and nanomaterials have proved to be excellent tools for in situ imaging of non-normal events such as abnormal pH and hypoxia in lesion areas. This chapter focuses on recent advances in the fluorescence imaging of tumour pH and hypoxia and their biological application in tumour therapeutic monitoring.