Non-exhaustive list of LRE-optimized 1H-detected experiments that have been proposed in the literature for NMR investigations of proteins or nucleic acids.
| LRE technique . | Application . | Purpose . | Refs. . |
|---|---|---|---|
| SOFAST | Proteins | 2D 1H–15N or 1H–13C | Schanda 200538,39 |
| Amero 200942 | |||
| ST-SOFAST | Proteins | 2D 1H–15N or 1H–13C | Kern 200848 |
| J-SOFAST | Proteins | 2D 1H–15N or 1H–13C | Kupce 200749 |
| Mueller 200850 | |||
| ultraSOFAST | Proteins | 2D 1H–15N or 1H–13C | Gal 200754 |
| Kern 200848 | |||
| SOFAST | RNA (iminos) | 2D 1H–15N | Farjon 200915 |
| SOFAST | RNA (bases) | 2D 1H–13C | Sathyamoorthy 201458 |
| BEST-HSQC | Proteins | 2D 1H–15N | Pervushin 200210 |
| BEST-HSQC | Proteins | 3D Backbone assignment expts: | Schanda 200616 |
| Lescop 200724 | |||
| HNCO, HNCA, HNCACB, HN(CO)CA, HN(CO)CACB, iHNCA, iHNCACB, HNN | |||
| Kumar 201059 | |||
| BEST-HSQC | Proteins | Backbone J coupling and RDC measurements: N–HN, Cα–Hα, CO–H HN, N–CO… | Rasia 201160 |
| BEST-HSQC | Proteins | Measurement of RDCs between amide 1H | Schanda 200761 |
| BEST-TROSY | Proteins | 2D 1H–15N | Pervushin 200210 |
| Favier 201125 | |||
| BEST-TROSY | RNA (iminos) | 2D 1H–15N | Farjon 200915 |
| BEST-TROSY | Proteins | Backbone assignment: | Pervushin 200210 |
| Favier 201125 | |||
| 3D HNCO, HNCA, HNCACB, HN(CO)CA, HN(CO)CACB, iHNCA, iHNCACB, (H)N(COCA)NH, (HN)CO(CA)NH | Solyom 201326 | ||
| BEST-TROSY | Proteins | Bidirectional HNC experiments with enhanced sequential correlation pathway: HNCA+, HNCO+, HNCACB+ | Gil-Caballero 201462 |
| BEST-TROSY | IDPs | Proline-selective 1H–15N correlation experiments | Solyom 201326 |
| BEST-TROSY | RNA (iminos) | Trans-hydrogen-bond correlation experiment: HNN-COSY | Farjon 200915 |
| HET-SOFAST/BEST | Proteins | Quantification of LRE effect from water exchange or 1H–1H cross-relaxation | Schanda 200657 |
| Rennella 201456 | |||
| Flip-back | Proteins | 2D 1H–15N | Deschamp 200663 |
| Flip-back | Proteins | Backbone assignment: | Diercks 200564 |
| 3D HNCO, HNCA | |||
| Flip-back | Proteins | Aromatic side chain assignment | Eletsky 200565 |
| LRE technique . | Application . | Purpose . | Refs. . |
|---|---|---|---|
| SOFAST | Proteins | 2D 1H–15N or 1H–13C | Schanda 200538,39 |
| Amero 200942 | |||
| ST-SOFAST | Proteins | 2D 1H–15N or 1H–13C | Kern 200848 |
| J-SOFAST | Proteins | 2D 1H–15N or 1H–13C | Kupce 200749 |
| Mueller 200850 | |||
| ultraSOFAST | Proteins | 2D 1H–15N or 1H–13C | Gal 200754 |
| Kern 200848 | |||
| SOFAST | RNA (iminos) | 2D 1H–15N | Farjon 200915 |
| SOFAST | RNA (bases) | 2D 1H–13C | Sathyamoorthy 201458 |
| BEST-HSQC | Proteins | 2D 1H–15N | Pervushin 200210 |
| BEST-HSQC | Proteins | 3D Backbone assignment expts: | Schanda 200616 |
| Lescop 200724 | |||
| HNCO, HNCA, HNCACB, HN(CO)CA, HN(CO)CACB, iHNCA, iHNCACB, HNN | |||
| Kumar 201059 | |||
| BEST-HSQC | Proteins | Backbone J coupling and RDC measurements: N–HN, Cα–Hα, CO–H HN, N–CO… | Rasia 201160 |
| BEST-HSQC | Proteins | Measurement of RDCs between amide 1H | Schanda 200761 |
| BEST-TROSY | Proteins | 2D 1H–15N | Pervushin 200210 |
| Favier 201125 | |||
| BEST-TROSY | RNA (iminos) | 2D 1H–15N | Farjon 200915 |
| BEST-TROSY | Proteins | Backbone assignment: | Pervushin 200210 |
| Favier 201125 | |||
| 3D HNCO, HNCA, HNCACB, HN(CO)CA, HN(CO)CACB, iHNCA, iHNCACB, (H)N(COCA)NH, (HN)CO(CA)NH | Solyom 201326 | ||
| BEST-TROSY | Proteins | Bidirectional HNC experiments with enhanced sequential correlation pathway: HNCA+, HNCO+, HNCACB+ | Gil-Caballero 201462 |
| BEST-TROSY | IDPs | Proline-selective 1H–15N correlation experiments | Solyom 201326 |
| BEST-TROSY | RNA (iminos) | Trans-hydrogen-bond correlation experiment: HNN-COSY | Farjon 200915 |
| HET-SOFAST/BEST | Proteins | Quantification of LRE effect from water exchange or 1H–1H cross-relaxation | Schanda 200657 |
| Rennella 201456 | |||
| Flip-back | Proteins | 2D 1H–15N | Deschamp 200663 |
| Flip-back | Proteins | Backbone assignment: | Diercks 200564 |
| 3D HNCO, HNCA | |||
| Flip-back | Proteins | Aromatic side chain assignment | Eletsky 200565 |